Nutrient agar rvfm 52 2601 nutrient agar is a general purpose growth medium suitable for use in a range of biological experiments use 28g powder to 1 litre deionised water leave for 10 minutes swirl and sterilise for 15 minutes at 121 deg c cool to 47 deg c mix well then pour into plates or petri dishes rvfm nutrient agar 100g